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il 6  (R&D Systems)


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    Structured Review

    R&D Systems il 6
    Il 6, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 960 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+il-6/Recombinant+Human+IL-6+Protein/us12606556-555-11-15
    Average 96 stars, based on 960 article reviews
    il 6 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Transfection:

    Article Title: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells
    Article Snippet: Transfected cells were cultured with recombinant human IL-6 (#206-IL-050, R&D) (10 ng/mL) for 12 h or 48 h.

    Article Title: IL-27 Is Elevated in Patients With COPD and Patients With Pulmonary TB and Induces Human Bronchial Epithelial Cells to Produce CXCL10
    Article Snippet: Recombinant human IL-1β, IL-4, IL-6, IL-12, IL-13, IL-17F, IL-23, IL-27, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were purchased from R&D Systems.

    Article Title: Antioxidant inflammation modulators: oleanolic acid derivatives with amino and other modifications at C-17
    Article Snippet: Lysates were immunoblotted with antibodies against phosphorylated or total STAT3 (Cell Signaling). iNOS Induction qPCR.

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100 μg/ml and stored at −20C.

    Article Title: Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.
    Article Snippet: From the University of California San Diego human research protection program) according to the protocol by Kirshenbaum and Metcalfe.59 Briefly, CD34+ cells were cultured in serum-free culture media (Stemline II, Sigma) containing antibiotic-antimycotic (Thermo Fisher), recombinant human stem cell factor (100 ng/ml, R&D Systems), recombinant human IL-6 (100 ng/ml, R&D Systems), and recombinant human IL-3 (20 ng/ml, R&D Systems, first week only).

    Article Title: IL-6 trans- signaling drives a STAT3-dependent pathway that leads to structural alterations of the peritoneal membrane.
    Article Snippet: Recombinant human IL-6, soluble IL-6 receptor (sIL-6R) and 158 soluble gp130 (sgp130) were purchased from R&D Systems 159 (Minneapolis, MN).The STAT3 specific inhibitor S3I-201 and the TGFβ1 160 specific inhibitor SB431542 were purchased from Merck Life Science 161 (Shanghai, China).

    Article Title: Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
    Article Snippet: Peptide, recombinant protein , Recombinant human IL-6 , R and D systems , Cat# 206-IL-010 , .

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes.
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100μg/ml and stored at −20C.

    Cell Culture:

    Article Title: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells
    Article Snippet: Transfected cells were cultured with recombinant human IL-6 (#206-IL-050, R&D) (10 ng/mL) for 12 h or 48 h.

    Article Title: IL-27 Is Elevated in Patients With COPD and Patients With Pulmonary TB and Induces Human Bronchial Epithelial Cells to Produce CXCL10
    Article Snippet: Recombinant human IL-1β, IL-4, IL-6, IL-12, IL-13, IL-17F, IL-23, IL-27, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were purchased from R&D Systems.

    Article Title: Antioxidant inflammation modulators: oleanolic acid derivatives with amino and other modifications at C-17
    Article Snippet: Lysates were immunoblotted with antibodies against phosphorylated or total STAT3 (Cell Signaling). iNOS Induction qPCR.

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100 μg/ml and stored at −20C.

    Article Title: Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.
    Article Snippet: From the University of California San Diego human research protection program) according to the protocol by Kirshenbaum and Metcalfe.59 Briefly, CD34+ cells were cultured in serum-free culture media (Stemline II, Sigma) containing antibiotic-antimycotic (Thermo Fisher), recombinant human stem cell factor (100 ng/ml, R&D Systems), recombinant human IL-6 (100 ng/ml, R&D Systems), and recombinant human IL-3 (20 ng/ml, R&D Systems, first week only).

    Article Title: IL-6 trans- signaling drives a STAT3-dependent pathway that leads to structural alterations of the peritoneal membrane.
    Article Snippet: Recombinant human IL-6, soluble IL-6 receptor (sIL-6R) and 158 soluble gp130 (sgp130) were purchased from R&D Systems 159 (Minneapolis, MN).The STAT3 specific inhibitor S3I-201 and the TGFβ1 160 specific inhibitor SB431542 were purchased from Merck Life Science 161 (Shanghai, China).

    Article Title: Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
    Article Snippet: Peptide, recombinant protein , Recombinant human IL-6 , R and D systems , Cat# 206-IL-010 , .

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes.
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100μg/ml and stored at −20C.

    Recombinant:

    Article Title: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells
    Article Snippet: Transfected cells were cultured with recombinant human IL-6 (#206-IL-050, R&D) (10 ng/mL) for 12 h or 48 h.

    Article Title: IL-27 Is Elevated in Patients With COPD and Patients With Pulmonary TB and Induces Human Bronchial Epithelial Cells to Produce CXCL10
    Article Snippet: Recombinant human IL-1β, IL-4, IL-6, IL-12, IL-13, IL-17F, IL-23, IL-27, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were purchased from R&D Systems.

    Article Title: Antioxidant inflammation modulators: oleanolic acid derivatives with amino and other modifications at C-17
    Article Snippet: Lysates were immunoblotted with antibodies against phosphorylated or total STAT3 (Cell Signaling). iNOS Induction qPCR.

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100 μg/ml and stored at −20C.

    Article Title: Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.
    Article Snippet: From the University of California San Diego human research protection program) according to the protocol by Kirshenbaum and Metcalfe.59 Briefly, CD34+ cells were cultured in serum-free culture media (Stemline II, Sigma) containing antibiotic-antimycotic (Thermo Fisher), recombinant human stem cell factor (100 ng/ml, R&D Systems), recombinant human IL-6 (100 ng/ml, R&D Systems), and recombinant human IL-3 (20 ng/ml, R&D Systems, first week only).

    Article Title: IL-6 trans- signaling drives a STAT3-dependent pathway that leads to structural alterations of the peritoneal membrane.
    Article Snippet: Recombinant human IL-6, soluble IL-6 receptor (sIL-6R) and 158 soluble gp130 (sgp130) were purchased from R&D Systems 159 (Minneapolis, MN).The STAT3 specific inhibitor S3I-201 and the TGFβ1 160 specific inhibitor SB431542 were purchased from Merck Life Science 161 (Shanghai, China).

    Article Title: Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
    Article Snippet: Peptide, recombinant protein , Recombinant human IL-6 , R and D systems , Cat# 206-IL-010 , .

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes.
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100μg/ml and stored at −20C.

    Sterility:

    Article Title: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells
    Article Snippet: Transfected cells were cultured with recombinant human IL-6 (#206-IL-050, R&D) (10 ng/mL) for 12 h or 48 h.

    Article Title: IL-27 Is Elevated in Patients With COPD and Patients With Pulmonary TB and Induces Human Bronchial Epithelial Cells to Produce CXCL10
    Article Snippet: Recombinant human IL-1β, IL-4, IL-6, IL-12, IL-13, IL-17F, IL-23, IL-27, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were purchased from R&D Systems.

    Article Title: Antioxidant inflammation modulators: oleanolic acid derivatives with amino and other modifications at C-17
    Article Snippet: Lysates were immunoblotted with antibodies against phosphorylated or total STAT3 (Cell Signaling). iNOS Induction qPCR.

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100 μg/ml and stored at −20C.

    Article Title: Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.
    Article Snippet: From the University of California San Diego human research protection program) according to the protocol by Kirshenbaum and Metcalfe.59 Briefly, CD34+ cells were cultured in serum-free culture media (Stemline II, Sigma) containing antibiotic-antimycotic (Thermo Fisher), recombinant human stem cell factor (100 ng/ml, R&D Systems), recombinant human IL-6 (100 ng/ml, R&D Systems), and recombinant human IL-3 (20 ng/ml, R&D Systems, first week only).

    Article Title: IL-6 trans- signaling drives a STAT3-dependent pathway that leads to structural alterations of the peritoneal membrane.
    Article Snippet: Recombinant human IL-6, soluble IL-6 receptor (sIL-6R) and 158 soluble gp130 (sgp130) were purchased from R&D Systems 159 (Minneapolis, MN).The STAT3 specific inhibitor S3I-201 and the TGFβ1 160 specific inhibitor SB431542 were purchased from Merck Life Science 161 (Shanghai, China).

    Article Title: Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
    Article Snippet: Peptide, recombinant protein , Recombinant human IL-6 , R and D systems , Cat# 206-IL-010 , .

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes.
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100μg/ml and stored at −20C.

    Construct:

    Article Title: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells
    Article Snippet: Transfected cells were cultured with recombinant human IL-6 (#206-IL-050, R&D) (10 ng/mL) for 12 h or 48 h.

    Article Title: IL-27 Is Elevated in Patients With COPD and Patients With Pulmonary TB and Induces Human Bronchial Epithelial Cells to Produce CXCL10
    Article Snippet: Recombinant human IL-1β, IL-4, IL-6, IL-12, IL-13, IL-17F, IL-23, IL-27, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were purchased from R&D Systems.

    Article Title: Antioxidant inflammation modulators: oleanolic acid derivatives with amino and other modifications at C-17
    Article Snippet: Lysates were immunoblotted with antibodies against phosphorylated or total STAT3 (Cell Signaling). iNOS Induction qPCR.

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100 μg/ml and stored at −20C.

    Article Title: Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.
    Article Snippet: From the University of California San Diego human research protection program) according to the protocol by Kirshenbaum and Metcalfe.59 Briefly, CD34+ cells were cultured in serum-free culture media (Stemline II, Sigma) containing antibiotic-antimycotic (Thermo Fisher), recombinant human stem cell factor (100 ng/ml, R&D Systems), recombinant human IL-6 (100 ng/ml, R&D Systems), and recombinant human IL-3 (20 ng/ml, R&D Systems, first week only).

    Article Title: IL-6 trans- signaling drives a STAT3-dependent pathway that leads to structural alterations of the peritoneal membrane.
    Article Snippet: Recombinant human IL-6, soluble IL-6 receptor (sIL-6R) and 158 soluble gp130 (sgp130) were purchased from R&D Systems 159 (Minneapolis, MN).The STAT3 specific inhibitor S3I-201 and the TGFβ1 160 specific inhibitor SB431542 were purchased from Merck Life Science 161 (Shanghai, China).

    Article Title: Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
    Article Snippet: Peptide, recombinant protein , Recombinant human IL-6 , R and D systems , Cat# 206-IL-010 , .

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes.
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100μg/ml and stored at −20C.

    Expressing:

    Article Title: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells
    Article Snippet: Transfected cells were cultured with recombinant human IL-6 (#206-IL-050, R&D) (10 ng/mL) for 12 h or 48 h.

    Article Title: IL-27 Is Elevated in Patients With COPD and Patients With Pulmonary TB and Induces Human Bronchial Epithelial Cells to Produce CXCL10
    Article Snippet: Recombinant human IL-1β, IL-4, IL-6, IL-12, IL-13, IL-17F, IL-23, IL-27, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were purchased from R&D Systems.

    Article Title: Antioxidant inflammation modulators: oleanolic acid derivatives with amino and other modifications at C-17
    Article Snippet: Lysates were immunoblotted with antibodies against phosphorylated or total STAT3 (Cell Signaling). iNOS Induction qPCR.

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100 μg/ml and stored at −20C.

    Article Title: Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.
    Article Snippet: From the University of California San Diego human research protection program) according to the protocol by Kirshenbaum and Metcalfe.59 Briefly, CD34+ cells were cultured in serum-free culture media (Stemline II, Sigma) containing antibiotic-antimycotic (Thermo Fisher), recombinant human stem cell factor (100 ng/ml, R&D Systems), recombinant human IL-6 (100 ng/ml, R&D Systems), and recombinant human IL-3 (20 ng/ml, R&D Systems, first week only).

    Article Title: IL-6 trans- signaling drives a STAT3-dependent pathway that leads to structural alterations of the peritoneal membrane.
    Article Snippet: Recombinant human IL-6, soluble IL-6 receptor (sIL-6R) and 158 soluble gp130 (sgp130) were purchased from R&D Systems 159 (Minneapolis, MN).The STAT3 specific inhibitor S3I-201 and the TGFβ1 160 specific inhibitor SB431542 were purchased from Merck Life Science 161 (Shanghai, China).

    Article Title: Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
    Article Snippet: Peptide, recombinant protein , Recombinant human IL-6 , R and D systems , Cat# 206-IL-010 , .

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes.
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100μg/ml and stored at −20C.

    Plasmid Preparation:

    Article Title: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells
    Article Snippet: Transfected cells were cultured with recombinant human IL-6 (#206-IL-050, R&D) (10 ng/mL) for 12 h or 48 h.

    Article Title: IL-27 Is Elevated in Patients With COPD and Patients With Pulmonary TB and Induces Human Bronchial Epithelial Cells to Produce CXCL10
    Article Snippet: Recombinant human IL-1β, IL-4, IL-6, IL-12, IL-13, IL-17F, IL-23, IL-27, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were purchased from R&D Systems.

    Article Title: Antioxidant inflammation modulators: oleanolic acid derivatives with amino and other modifications at C-17
    Article Snippet: Lysates were immunoblotted with antibodies against phosphorylated or total STAT3 (Cell Signaling). iNOS Induction qPCR.

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100 μg/ml and stored at −20C.

    Article Title: Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.
    Article Snippet: From the University of California San Diego human research protection program) according to the protocol by Kirshenbaum and Metcalfe.59 Briefly, CD34+ cells were cultured in serum-free culture media (Stemline II, Sigma) containing antibiotic-antimycotic (Thermo Fisher), recombinant human stem cell factor (100 ng/ml, R&D Systems), recombinant human IL-6 (100 ng/ml, R&D Systems), and recombinant human IL-3 (20 ng/ml, R&D Systems, first week only).

    Article Title: IL-6 trans- signaling drives a STAT3-dependent pathway that leads to structural alterations of the peritoneal membrane.
    Article Snippet: Recombinant human IL-6, soluble IL-6 receptor (sIL-6R) and 158 soluble gp130 (sgp130) were purchased from R&D Systems 159 (Minneapolis, MN).The STAT3 specific inhibitor S3I-201 and the TGFβ1 160 specific inhibitor SB431542 were purchased from Merck Life Science 161 (Shanghai, China).

    Article Title: Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
    Article Snippet: Peptide, recombinant protein , Recombinant human IL-6 , R and D systems , Cat# 206-IL-010 , .

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes.
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100μg/ml and stored at −20C.

    Neutralization:

    Article Title: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells
    Article Snippet: Transfected cells were cultured with recombinant human IL-6 (#206-IL-050, R&D) (10 ng/mL) for 12 h or 48 h.

    Article Title: IL-27 Is Elevated in Patients With COPD and Patients With Pulmonary TB and Induces Human Bronchial Epithelial Cells to Produce CXCL10
    Article Snippet: Recombinant human IL-1β, IL-4, IL-6, IL-12, IL-13, IL-17F, IL-23, IL-27, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were purchased from R&D Systems.

    Article Title: Antioxidant inflammation modulators: oleanolic acid derivatives with amino and other modifications at C-17
    Article Snippet: Lysates were immunoblotted with antibodies against phosphorylated or total STAT3 (Cell Signaling). iNOS Induction qPCR.

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100 μg/ml and stored at −20C.

    Article Title: Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.
    Article Snippet: From the University of California San Diego human research protection program) according to the protocol by Kirshenbaum and Metcalfe.59 Briefly, CD34+ cells were cultured in serum-free culture media (Stemline II, Sigma) containing antibiotic-antimycotic (Thermo Fisher), recombinant human stem cell factor (100 ng/ml, R&D Systems), recombinant human IL-6 (100 ng/ml, R&D Systems), and recombinant human IL-3 (20 ng/ml, R&D Systems, first week only).

    Article Title: IL-6 trans- signaling drives a STAT3-dependent pathway that leads to structural alterations of the peritoneal membrane.
    Article Snippet: Recombinant human IL-6, soluble IL-6 receptor (sIL-6R) and 158 soluble gp130 (sgp130) were purchased from R&D Systems 159 (Minneapolis, MN).The STAT3 specific inhibitor S3I-201 and the TGFβ1 160 specific inhibitor SB431542 were purchased from Merck Life Science 161 (Shanghai, China).

    Article Title: Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
    Article Snippet: Peptide, recombinant protein , Recombinant human IL-6 , R and D systems , Cat# 206-IL-010 , .

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes.
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100μg/ml and stored at −20C.

    Control:

    Article Title: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells
    Article Snippet: Transfected cells were cultured with recombinant human IL-6 (#206-IL-050, R&D) (10 ng/mL) for 12 h or 48 h.

    Article Title: IL-27 Is Elevated in Patients With COPD and Patients With Pulmonary TB and Induces Human Bronchial Epithelial Cells to Produce CXCL10
    Article Snippet: Recombinant human IL-1β, IL-4, IL-6, IL-12, IL-13, IL-17F, IL-23, IL-27, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were purchased from R&D Systems.

    Article Title: Antioxidant inflammation modulators: oleanolic acid derivatives with amino and other modifications at C-17
    Article Snippet: Lysates were immunoblotted with antibodies against phosphorylated or total STAT3 (Cell Signaling). iNOS Induction qPCR.

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100 μg/ml and stored at −20C.

    Article Title: Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.
    Article Snippet: From the University of California San Diego human research protection program) according to the protocol by Kirshenbaum and Metcalfe.59 Briefly, CD34+ cells were cultured in serum-free culture media (Stemline II, Sigma) containing antibiotic-antimycotic (Thermo Fisher), recombinant human stem cell factor (100 ng/ml, R&D Systems), recombinant human IL-6 (100 ng/ml, R&D Systems), and recombinant human IL-3 (20 ng/ml, R&D Systems, first week only).

    Article Title: IL-6 trans- signaling drives a STAT3-dependent pathway that leads to structural alterations of the peritoneal membrane.
    Article Snippet: Recombinant human IL-6, soluble IL-6 receptor (sIL-6R) and 158 soluble gp130 (sgp130) were purchased from R&D Systems 159 (Minneapolis, MN).The STAT3 specific inhibitor S3I-201 and the TGFβ1 160 specific inhibitor SB431542 were purchased from Merck Life Science 161 (Shanghai, China).

    Article Title: Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
    Article Snippet: Peptide, recombinant protein , Recombinant human IL-6 , R and D systems , Cat# 206-IL-010 , .

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes.
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100μg/ml and stored at −20C.

    Functional Assay:

    Article Title: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells
    Article Snippet: Transfected cells were cultured with recombinant human IL-6 (#206-IL-050, R&D) (10 ng/mL) for 12 h or 48 h.

    Article Title: IL-27 Is Elevated in Patients With COPD and Patients With Pulmonary TB and Induces Human Bronchial Epithelial Cells to Produce CXCL10
    Article Snippet: Recombinant human IL-1β, IL-4, IL-6, IL-12, IL-13, IL-17F, IL-23, IL-27, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were purchased from R&D Systems.

    Article Title: Antioxidant inflammation modulators: oleanolic acid derivatives with amino and other modifications at C-17
    Article Snippet: Lysates were immunoblotted with antibodies against phosphorylated or total STAT3 (Cell Signaling). iNOS Induction qPCR.

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100 μg/ml and stored at −20C.

    Article Title: Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.
    Article Snippet: From the University of California San Diego human research protection program) according to the protocol by Kirshenbaum and Metcalfe.59 Briefly, CD34+ cells were cultured in serum-free culture media (Stemline II, Sigma) containing antibiotic-antimycotic (Thermo Fisher), recombinant human stem cell factor (100 ng/ml, R&D Systems), recombinant human IL-6 (100 ng/ml, R&D Systems), and recombinant human IL-3 (20 ng/ml, R&D Systems, first week only).

    Article Title: IL-6 trans- signaling drives a STAT3-dependent pathway that leads to structural alterations of the peritoneal membrane.
    Article Snippet: Recombinant human IL-6, soluble IL-6 receptor (sIL-6R) and 158 soluble gp130 (sgp130) were purchased from R&D Systems 159 (Minneapolis, MN).The STAT3 specific inhibitor S3I-201 and the TGFβ1 160 specific inhibitor SB431542 were purchased from Merck Life Science 161 (Shanghai, China).

    Article Title: Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
    Article Snippet: Peptide, recombinant protein , Recombinant human IL-6 , R and D systems , Cat# 206-IL-010 , .

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes.
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100μg/ml and stored at −20C.

    Purification:

    Article Title: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells
    Article Snippet: Transfected cells were cultured with recombinant human IL-6 (#206-IL-050, R&D) (10 ng/mL) for 12 h or 48 h.

    Article Title: IL-27 Is Elevated in Patients With COPD and Patients With Pulmonary TB and Induces Human Bronchial Epithelial Cells to Produce CXCL10
    Article Snippet: Recombinant human IL-1β, IL-4, IL-6, IL-12, IL-13, IL-17F, IL-23, IL-27, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were purchased from R&D Systems.

    Article Title: Antioxidant inflammation modulators: oleanolic acid derivatives with amino and other modifications at C-17
    Article Snippet: Lysates were immunoblotted with antibodies against phosphorylated or total STAT3 (Cell Signaling). iNOS Induction qPCR.

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100 μg/ml and stored at −20C.

    Article Title: Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.
    Article Snippet: From the University of California San Diego human research protection program) according to the protocol by Kirshenbaum and Metcalfe.59 Briefly, CD34+ cells were cultured in serum-free culture media (Stemline II, Sigma) containing antibiotic-antimycotic (Thermo Fisher), recombinant human stem cell factor (100 ng/ml, R&D Systems), recombinant human IL-6 (100 ng/ml, R&D Systems), and recombinant human IL-3 (20 ng/ml, R&D Systems, first week only).

    Article Title: IL-6 trans- signaling drives a STAT3-dependent pathway that leads to structural alterations of the peritoneal membrane.
    Article Snippet: Recombinant human IL-6, soluble IL-6 receptor (sIL-6R) and 158 soluble gp130 (sgp130) were purchased from R&D Systems 159 (Minneapolis, MN).The STAT3 specific inhibitor S3I-201 and the TGFβ1 160 specific inhibitor SB431542 were purchased from Merck Life Science 161 (Shanghai, China).

    Article Title: Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
    Article Snippet: Peptide, recombinant protein , Recombinant human IL-6 , R and D systems , Cat# 206-IL-010 , .

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes.
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100μg/ml and stored at −20C.

    Luciferase:

    Article Title: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells
    Article Snippet: Transfected cells were cultured with recombinant human IL-6 (#206-IL-050, R&D) (10 ng/mL) for 12 h or 48 h.

    Article Title: IL-27 Is Elevated in Patients With COPD and Patients With Pulmonary TB and Induces Human Bronchial Epithelial Cells to Produce CXCL10
    Article Snippet: Recombinant human IL-1β, IL-4, IL-6, IL-12, IL-13, IL-17F, IL-23, IL-27, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were purchased from R&D Systems.

    Article Title: Antioxidant inflammation modulators: oleanolic acid derivatives with amino and other modifications at C-17
    Article Snippet: Lysates were immunoblotted with antibodies against phosphorylated or total STAT3 (Cell Signaling). iNOS Induction qPCR.

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100 μg/ml and stored at −20C.

    Article Title: Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.
    Article Snippet: From the University of California San Diego human research protection program) according to the protocol by Kirshenbaum and Metcalfe.59 Briefly, CD34+ cells were cultured in serum-free culture media (Stemline II, Sigma) containing antibiotic-antimycotic (Thermo Fisher), recombinant human stem cell factor (100 ng/ml, R&D Systems), recombinant human IL-6 (100 ng/ml, R&D Systems), and recombinant human IL-3 (20 ng/ml, R&D Systems, first week only).

    Article Title: IL-6 trans- signaling drives a STAT3-dependent pathway that leads to structural alterations of the peritoneal membrane.
    Article Snippet: Recombinant human IL-6, soluble IL-6 receptor (sIL-6R) and 158 soluble gp130 (sgp130) were purchased from R&D Systems 159 (Minneapolis, MN).The STAT3 specific inhibitor S3I-201 and the TGFβ1 160 specific inhibitor SB431542 were purchased from Merck Life Science 161 (Shanghai, China).

    Article Title: Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
    Article Snippet: Peptide, recombinant protein , Recombinant human IL-6 , R and D systems , Cat# 206-IL-010 , .

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes.
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100μg/ml and stored at −20C.

    Over Expression:

    Article Title: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells
    Article Snippet: Transfected cells were cultured with recombinant human IL-6 (#206-IL-050, R&D) (10 ng/mL) for 12 h or 48 h.

    Article Title: IL-27 Is Elevated in Patients With COPD and Patients With Pulmonary TB and Induces Human Bronchial Epithelial Cells to Produce CXCL10
    Article Snippet: Recombinant human IL-1β, IL-4, IL-6, IL-12, IL-13, IL-17F, IL-23, IL-27, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were purchased from R&D Systems.

    Article Title: Antioxidant inflammation modulators: oleanolic acid derivatives with amino and other modifications at C-17
    Article Snippet: Lysates were immunoblotted with antibodies against phosphorylated or total STAT3 (Cell Signaling). iNOS Induction qPCR.

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100 μg/ml and stored at −20C.

    Article Title: Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.
    Article Snippet: From the University of California San Diego human research protection program) according to the protocol by Kirshenbaum and Metcalfe.59 Briefly, CD34+ cells were cultured in serum-free culture media (Stemline II, Sigma) containing antibiotic-antimycotic (Thermo Fisher), recombinant human stem cell factor (100 ng/ml, R&D Systems), recombinant human IL-6 (100 ng/ml, R&D Systems), and recombinant human IL-3 (20 ng/ml, R&D Systems, first week only).

    Article Title: IL-6 trans- signaling drives a STAT3-dependent pathway that leads to structural alterations of the peritoneal membrane.
    Article Snippet: Recombinant human IL-6, soluble IL-6 receptor (sIL-6R) and 158 soluble gp130 (sgp130) were purchased from R&D Systems 159 (Minneapolis, MN).The STAT3 specific inhibitor S3I-201 and the TGFβ1 160 specific inhibitor SB431542 were purchased from Merck Life Science 161 (Shanghai, China).

    Article Title: Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
    Article Snippet: Peptide, recombinant protein , Recombinant human IL-6 , R and D systems , Cat# 206-IL-010 , .

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes.
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100μg/ml and stored at −20C.

    Inhibition:

    Article Title: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells
    Article Snippet: Transfected cells were cultured with recombinant human IL-6 (#206-IL-050, R&D) (10 ng/mL) for 12 h or 48 h.

    Article Title: IL-27 Is Elevated in Patients With COPD and Patients With Pulmonary TB and Induces Human Bronchial Epithelial Cells to Produce CXCL10
    Article Snippet: Recombinant human IL-1β, IL-4, IL-6, IL-12, IL-13, IL-17F, IL-23, IL-27, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were purchased from R&D Systems.

    Article Title: Antioxidant inflammation modulators: oleanolic acid derivatives with amino and other modifications at C-17
    Article Snippet: Lysates were immunoblotted with antibodies against phosphorylated or total STAT3 (Cell Signaling). iNOS Induction qPCR.

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100 μg/ml and stored at −20C.

    Article Title: Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.
    Article Snippet: From the University of California San Diego human research protection program) according to the protocol by Kirshenbaum and Metcalfe.59 Briefly, CD34+ cells were cultured in serum-free culture media (Stemline II, Sigma) containing antibiotic-antimycotic (Thermo Fisher), recombinant human stem cell factor (100 ng/ml, R&D Systems), recombinant human IL-6 (100 ng/ml, R&D Systems), and recombinant human IL-3 (20 ng/ml, R&D Systems, first week only).

    Article Title: IL-6 trans- signaling drives a STAT3-dependent pathway that leads to structural alterations of the peritoneal membrane.
    Article Snippet: Recombinant human IL-6, soluble IL-6 receptor (sIL-6R) and 158 soluble gp130 (sgp130) were purchased from R&D Systems 159 (Minneapolis, MN).The STAT3 specific inhibitor S3I-201 and the TGFβ1 160 specific inhibitor SB431542 were purchased from Merck Life Science 161 (Shanghai, China).

    Article Title: Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
    Article Snippet: Peptide, recombinant protein , Recombinant human IL-6 , R and D systems , Cat# 206-IL-010 , .

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes.
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100μg/ml and stored at −20C.

    Mutagenesis:

    Article Title: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells
    Article Snippet: Transfected cells were cultured with recombinant human IL-6 (#206-IL-050, R&D) (10 ng/mL) for 12 h or 48 h.

    Article Title: IL-27 Is Elevated in Patients With COPD and Patients With Pulmonary TB and Induces Human Bronchial Epithelial Cells to Produce CXCL10
    Article Snippet: Recombinant human IL-1β, IL-4, IL-6, IL-12, IL-13, IL-17F, IL-23, IL-27, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were purchased from R&D Systems.

    Article Title: Antioxidant inflammation modulators: oleanolic acid derivatives with amino and other modifications at C-17
    Article Snippet: Lysates were immunoblotted with antibodies against phosphorylated or total STAT3 (Cell Signaling). iNOS Induction qPCR.

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100 μg/ml and stored at −20C.

    Article Title: Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.
    Article Snippet: From the University of California San Diego human research protection program) according to the protocol by Kirshenbaum and Metcalfe.59 Briefly, CD34+ cells were cultured in serum-free culture media (Stemline II, Sigma) containing antibiotic-antimycotic (Thermo Fisher), recombinant human stem cell factor (100 ng/ml, R&D Systems), recombinant human IL-6 (100 ng/ml, R&D Systems), and recombinant human IL-3 (20 ng/ml, R&D Systems, first week only).

    Article Title: IL-6 trans- signaling drives a STAT3-dependent pathway that leads to structural alterations of the peritoneal membrane.
    Article Snippet: Recombinant human IL-6, soluble IL-6 receptor (sIL-6R) and 158 soluble gp130 (sgp130) were purchased from R&D Systems 159 (Minneapolis, MN).The STAT3 specific inhibitor S3I-201 and the TGFβ1 160 specific inhibitor SB431542 were purchased from Merck Life Science 161 (Shanghai, China).

    Article Title: Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
    Article Snippet: Peptide, recombinant protein , Recombinant human IL-6 , R and D systems , Cat# 206-IL-010 , .

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes.
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100μg/ml and stored at −20C.

    Cell Isolation:

    Article Title: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells
    Article Snippet: Transfected cells were cultured with recombinant human IL-6 (#206-IL-050, R&D) (10 ng/mL) for 12 h or 48 h.

    Article Title: IL-27 Is Elevated in Patients With COPD and Patients With Pulmonary TB and Induces Human Bronchial Epithelial Cells to Produce CXCL10
    Article Snippet: Recombinant human IL-1β, IL-4, IL-6, IL-12, IL-13, IL-17F, IL-23, IL-27, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were purchased from R&D Systems.

    Article Title: Antioxidant inflammation modulators: oleanolic acid derivatives with amino and other modifications at C-17
    Article Snippet: Lysates were immunoblotted with antibodies against phosphorylated or total STAT3 (Cell Signaling). iNOS Induction qPCR.

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100 μg/ml and stored at −20C.

    Article Title: Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.
    Article Snippet: From the University of California San Diego human research protection program) according to the protocol by Kirshenbaum and Metcalfe.59 Briefly, CD34+ cells were cultured in serum-free culture media (Stemline II, Sigma) containing antibiotic-antimycotic (Thermo Fisher), recombinant human stem cell factor (100 ng/ml, R&D Systems), recombinant human IL-6 (100 ng/ml, R&D Systems), and recombinant human IL-3 (20 ng/ml, R&D Systems, first week only).

    Article Title: IL-6 trans- signaling drives a STAT3-dependent pathway that leads to structural alterations of the peritoneal membrane.
    Article Snippet: Recombinant human IL-6, soluble IL-6 receptor (sIL-6R) and 158 soluble gp130 (sgp130) were purchased from R&D Systems 159 (Minneapolis, MN).The STAT3 specific inhibitor S3I-201 and the TGFβ1 160 specific inhibitor SB431542 were purchased from Merck Life Science 161 (Shanghai, China).

    Article Title: Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
    Article Snippet: Peptide, recombinant protein , Recombinant human IL-6 , R and D systems , Cat# 206-IL-010 , .

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes.
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100μg/ml and stored at −20C.

    Activation Assay:

    Article Title: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells
    Article Snippet: Transfected cells were cultured with recombinant human IL-6 (#206-IL-050, R&D) (10 ng/mL) for 12 h or 48 h.

    Article Title: IL-27 Is Elevated in Patients With COPD and Patients With Pulmonary TB and Induces Human Bronchial Epithelial Cells to Produce CXCL10
    Article Snippet: Recombinant human IL-1β, IL-4, IL-6, IL-12, IL-13, IL-17F, IL-23, IL-27, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were purchased from R&D Systems.

    Article Title: Antioxidant inflammation modulators: oleanolic acid derivatives with amino and other modifications at C-17
    Article Snippet: Lysates were immunoblotted with antibodies against phosphorylated or total STAT3 (Cell Signaling). iNOS Induction qPCR.

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100 μg/ml and stored at −20C.

    Article Title: Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.
    Article Snippet: From the University of California San Diego human research protection program) according to the protocol by Kirshenbaum and Metcalfe.59 Briefly, CD34+ cells were cultured in serum-free culture media (Stemline II, Sigma) containing antibiotic-antimycotic (Thermo Fisher), recombinant human stem cell factor (100 ng/ml, R&D Systems), recombinant human IL-6 (100 ng/ml, R&D Systems), and recombinant human IL-3 (20 ng/ml, R&D Systems, first week only).

    Article Title: IL-6 trans- signaling drives a STAT3-dependent pathway that leads to structural alterations of the peritoneal membrane.
    Article Snippet: Recombinant human IL-6, soluble IL-6 receptor (sIL-6R) and 158 soluble gp130 (sgp130) were purchased from R&D Systems 159 (Minneapolis, MN).The STAT3 specific inhibitor S3I-201 and the TGFβ1 160 specific inhibitor SB431542 were purchased from Merck Life Science 161 (Shanghai, China).

    Article Title: Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
    Article Snippet: Peptide, recombinant protein , Recombinant human IL-6 , R and D systems , Cat# 206-IL-010 , .

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes.
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100μg/ml and stored at −20C.

    Reporter Assay:

    Article Title: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells
    Article Snippet: Transfected cells were cultured with recombinant human IL-6 (#206-IL-050, R&D) (10 ng/mL) for 12 h or 48 h.

    Article Title: IL-27 Is Elevated in Patients With COPD and Patients With Pulmonary TB and Induces Human Bronchial Epithelial Cells to Produce CXCL10
    Article Snippet: Recombinant human IL-1β, IL-4, IL-6, IL-12, IL-13, IL-17F, IL-23, IL-27, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were purchased from R&D Systems.

    Article Title: Antioxidant inflammation modulators: oleanolic acid derivatives with amino and other modifications at C-17
    Article Snippet: Lysates were immunoblotted with antibodies against phosphorylated or total STAT3 (Cell Signaling). iNOS Induction qPCR.

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100 μg/ml and stored at −20C.

    Article Title: Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.
    Article Snippet: From the University of California San Diego human research protection program) according to the protocol by Kirshenbaum and Metcalfe.59 Briefly, CD34+ cells were cultured in serum-free culture media (Stemline II, Sigma) containing antibiotic-antimycotic (Thermo Fisher), recombinant human stem cell factor (100 ng/ml, R&D Systems), recombinant human IL-6 (100 ng/ml, R&D Systems), and recombinant human IL-3 (20 ng/ml, R&D Systems, first week only).

    Article Title: IL-6 trans- signaling drives a STAT3-dependent pathway that leads to structural alterations of the peritoneal membrane.
    Article Snippet: Recombinant human IL-6, soluble IL-6 receptor (sIL-6R) and 158 soluble gp130 (sgp130) were purchased from R&D Systems 159 (Minneapolis, MN).The STAT3 specific inhibitor S3I-201 and the TGFβ1 160 specific inhibitor SB431542 were purchased from Merck Life Science 161 (Shanghai, China).

    Article Title: Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
    Article Snippet: Peptide, recombinant protein , Recombinant human IL-6 , R and D systems , Cat# 206-IL-010 , .

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes.
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100μg/ml and stored at −20C.

    Reverse Transcription:

    Article Title: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells
    Article Snippet: Transfected cells were cultured with recombinant human IL-6 (#206-IL-050, R&D) (10 ng/mL) for 12 h or 48 h.

    Article Title: IL-27 Is Elevated in Patients With COPD and Patients With Pulmonary TB and Induces Human Bronchial Epithelial Cells to Produce CXCL10
    Article Snippet: Recombinant human IL-1β, IL-4, IL-6, IL-12, IL-13, IL-17F, IL-23, IL-27, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were purchased from R&D Systems.

    Article Title: Antioxidant inflammation modulators: oleanolic acid derivatives with amino and other modifications at C-17
    Article Snippet: Lysates were immunoblotted with antibodies against phosphorylated or total STAT3 (Cell Signaling). iNOS Induction qPCR.

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100 μg/ml and stored at −20C.

    Article Title: Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.
    Article Snippet: From the University of California San Diego human research protection program) according to the protocol by Kirshenbaum and Metcalfe.59 Briefly, CD34+ cells were cultured in serum-free culture media (Stemline II, Sigma) containing antibiotic-antimycotic (Thermo Fisher), recombinant human stem cell factor (100 ng/ml, R&D Systems), recombinant human IL-6 (100 ng/ml, R&D Systems), and recombinant human IL-3 (20 ng/ml, R&D Systems, first week only).

    Article Title: IL-6 trans- signaling drives a STAT3-dependent pathway that leads to structural alterations of the peritoneal membrane.
    Article Snippet: Recombinant human IL-6, soluble IL-6 receptor (sIL-6R) and 158 soluble gp130 (sgp130) were purchased from R&D Systems 159 (Minneapolis, MN).The STAT3 specific inhibitor S3I-201 and the TGFβ1 160 specific inhibitor SB431542 were purchased from Merck Life Science 161 (Shanghai, China).

    Article Title: Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
    Article Snippet: Peptide, recombinant protein , Recombinant human IL-6 , R and D systems , Cat# 206-IL-010 , .

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes.
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100μg/ml and stored at −20C.

    SYBR Green Assay:

    Article Title: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells
    Article Snippet: Transfected cells were cultured with recombinant human IL-6 (#206-IL-050, R&D) (10 ng/mL) for 12 h or 48 h.

    Article Title: IL-27 Is Elevated in Patients With COPD and Patients With Pulmonary TB and Induces Human Bronchial Epithelial Cells to Produce CXCL10
    Article Snippet: Recombinant human IL-1β, IL-4, IL-6, IL-12, IL-13, IL-17F, IL-23, IL-27, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were purchased from R&D Systems.

    Article Title: Antioxidant inflammation modulators: oleanolic acid derivatives with amino and other modifications at C-17
    Article Snippet: Lysates were immunoblotted with antibodies against phosphorylated or total STAT3 (Cell Signaling). iNOS Induction qPCR.

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100 μg/ml and stored at −20C.

    Article Title: Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.
    Article Snippet: From the University of California San Diego human research protection program) according to the protocol by Kirshenbaum and Metcalfe.59 Briefly, CD34+ cells were cultured in serum-free culture media (Stemline II, Sigma) containing antibiotic-antimycotic (Thermo Fisher), recombinant human stem cell factor (100 ng/ml, R&D Systems), recombinant human IL-6 (100 ng/ml, R&D Systems), and recombinant human IL-3 (20 ng/ml, R&D Systems, first week only).

    Article Title: IL-6 trans- signaling drives a STAT3-dependent pathway that leads to structural alterations of the peritoneal membrane.
    Article Snippet: Recombinant human IL-6, soluble IL-6 receptor (sIL-6R) and 158 soluble gp130 (sgp130) were purchased from R&D Systems 159 (Minneapolis, MN).The STAT3 specific inhibitor S3I-201 and the TGFβ1 160 specific inhibitor SB431542 were purchased from Merck Life Science 161 (Shanghai, China).

    Article Title: Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
    Article Snippet: Peptide, recombinant protein , Recombinant human IL-6 , R and D systems , Cat# 206-IL-010 , .

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes.
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100μg/ml and stored at −20C.

    Enzyme-linked Immunosorbent Assay:

    Article Title: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells
    Article Snippet: Transfected cells were cultured with recombinant human IL-6 (#206-IL-050, R&D) (10 ng/mL) for 12 h or 48 h.

    Article Title: IL-27 Is Elevated in Patients With COPD and Patients With Pulmonary TB and Induces Human Bronchial Epithelial Cells to Produce CXCL10
    Article Snippet: Recombinant human IL-1β, IL-4, IL-6, IL-12, IL-13, IL-17F, IL-23, IL-27, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were purchased from R&D Systems.

    Article Title: Antioxidant inflammation modulators: oleanolic acid derivatives with amino and other modifications at C-17
    Article Snippet: Lysates were immunoblotted with antibodies against phosphorylated or total STAT3 (Cell Signaling). iNOS Induction qPCR.

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100 μg/ml and stored at −20C.

    Article Title: Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.
    Article Snippet: From the University of California San Diego human research protection program) according to the protocol by Kirshenbaum and Metcalfe.59 Briefly, CD34+ cells were cultured in serum-free culture media (Stemline II, Sigma) containing antibiotic-antimycotic (Thermo Fisher), recombinant human stem cell factor (100 ng/ml, R&D Systems), recombinant human IL-6 (100 ng/ml, R&D Systems), and recombinant human IL-3 (20 ng/ml, R&D Systems, first week only).

    Article Title: IL-6 trans- signaling drives a STAT3-dependent pathway that leads to structural alterations of the peritoneal membrane.
    Article Snippet: Recombinant human IL-6, soluble IL-6 receptor (sIL-6R) and 158 soluble gp130 (sgp130) were purchased from R&D Systems 159 (Minneapolis, MN).The STAT3 specific inhibitor S3I-201 and the TGFβ1 160 specific inhibitor SB431542 were purchased from Merck Life Science 161 (Shanghai, China).

    Article Title: Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
    Article Snippet: Peptide, recombinant protein , Recombinant human IL-6 , R and D systems , Cat# 206-IL-010 , .

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes.
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100μg/ml and stored at −20C.

    Sequencing:

    Article Title: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells
    Article Snippet: Transfected cells were cultured with recombinant human IL-6 (#206-IL-050, R&D) (10 ng/mL) for 12 h or 48 h.

    Article Title: IL-27 Is Elevated in Patients With COPD and Patients With Pulmonary TB and Induces Human Bronchial Epithelial Cells to Produce CXCL10
    Article Snippet: Recombinant human IL-1β, IL-4, IL-6, IL-12, IL-13, IL-17F, IL-23, IL-27, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were purchased from R&D Systems.

    Article Title: Antioxidant inflammation modulators: oleanolic acid derivatives with amino and other modifications at C-17
    Article Snippet: Lysates were immunoblotted with antibodies against phosphorylated or total STAT3 (Cell Signaling). iNOS Induction qPCR.

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100 μg/ml and stored at −20C.

    Article Title: Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.
    Article Snippet: From the University of California San Diego human research protection program) according to the protocol by Kirshenbaum and Metcalfe.59 Briefly, CD34+ cells were cultured in serum-free culture media (Stemline II, Sigma) containing antibiotic-antimycotic (Thermo Fisher), recombinant human stem cell factor (100 ng/ml, R&D Systems), recombinant human IL-6 (100 ng/ml, R&D Systems), and recombinant human IL-3 (20 ng/ml, R&D Systems, first week only).

    Article Title: IL-6 trans- signaling drives a STAT3-dependent pathway that leads to structural alterations of the peritoneal membrane.
    Article Snippet: Recombinant human IL-6, soluble IL-6 receptor (sIL-6R) and 158 soluble gp130 (sgp130) were purchased from R&D Systems 159 (Minneapolis, MN).The STAT3 specific inhibitor S3I-201 and the TGFβ1 160 specific inhibitor SB431542 were purchased from Merck Life Science 161 (Shanghai, China).

    Article Title: Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
    Article Snippet: Peptide, recombinant protein , Recombinant human IL-6 , R and D systems , Cat# 206-IL-010 , .

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes.
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100μg/ml and stored at −20C.

    Software:

    Article Title: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells
    Article Snippet: Transfected cells were cultured with recombinant human IL-6 (#206-IL-050, R&D) (10 ng/mL) for 12 h or 48 h.

    Article Title: IL-27 Is Elevated in Patients With COPD and Patients With Pulmonary TB and Induces Human Bronchial Epithelial Cells to Produce CXCL10
    Article Snippet: Recombinant human IL-1β, IL-4, IL-6, IL-12, IL-13, IL-17F, IL-23, IL-27, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were purchased from R&D Systems.

    Article Title: Antioxidant inflammation modulators: oleanolic acid derivatives with amino and other modifications at C-17
    Article Snippet: Lysates were immunoblotted with antibodies against phosphorylated or total STAT3 (Cell Signaling). iNOS Induction qPCR.

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100 μg/ml and stored at −20C.

    Article Title: Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.
    Article Snippet: From the University of California San Diego human research protection program) according to the protocol by Kirshenbaum and Metcalfe.59 Briefly, CD34+ cells were cultured in serum-free culture media (Stemline II, Sigma) containing antibiotic-antimycotic (Thermo Fisher), recombinant human stem cell factor (100 ng/ml, R&D Systems), recombinant human IL-6 (100 ng/ml, R&D Systems), and recombinant human IL-3 (20 ng/ml, R&D Systems, first week only).

    Article Title: IL-6 trans- signaling drives a STAT3-dependent pathway that leads to structural alterations of the peritoneal membrane.
    Article Snippet: Recombinant human IL-6, soluble IL-6 receptor (sIL-6R) and 158 soluble gp130 (sgp130) were purchased from R&D Systems 159 (Minneapolis, MN).The STAT3 specific inhibitor S3I-201 and the TGFβ1 160 specific inhibitor SB431542 were purchased from Merck Life Science 161 (Shanghai, China).

    Article Title: Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
    Article Snippet: Peptide, recombinant protein , Recombinant human IL-6 , R and D systems , Cat# 206-IL-010 , .

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes.
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100μg/ml and stored at −20C.

    Sandwich ELISA:

    Article Title: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells
    Article Snippet: Transfected cells were cultured with recombinant human IL-6 (#206-IL-050, R&D) (10 ng/mL) for 12 h or 48 h.

    Article Title: IL-27 Is Elevated in Patients With COPD and Patients With Pulmonary TB and Induces Human Bronchial Epithelial Cells to Produce CXCL10
    Article Snippet: Recombinant human IL-1β, IL-4, IL-6, IL-12, IL-13, IL-17F, IL-23, IL-27, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were purchased from R&D Systems.

    Article Title: Antioxidant inflammation modulators: oleanolic acid derivatives with amino and other modifications at C-17
    Article Snippet: Lysates were immunoblotted with antibodies against phosphorylated or total STAT3 (Cell Signaling). iNOS Induction qPCR.

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100 μg/ml and stored at −20C.

    Article Title: Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.
    Article Snippet: From the University of California San Diego human research protection program) according to the protocol by Kirshenbaum and Metcalfe.59 Briefly, CD34+ cells were cultured in serum-free culture media (Stemline II, Sigma) containing antibiotic-antimycotic (Thermo Fisher), recombinant human stem cell factor (100 ng/ml, R&D Systems), recombinant human IL-6 (100 ng/ml, R&D Systems), and recombinant human IL-3 (20 ng/ml, R&D Systems, first week only).

    Article Title: IL-6 trans- signaling drives a STAT3-dependent pathway that leads to structural alterations of the peritoneal membrane.
    Article Snippet: Recombinant human IL-6, soluble IL-6 receptor (sIL-6R) and 158 soluble gp130 (sgp130) were purchased from R&D Systems 159 (Minneapolis, MN).The STAT3 specific inhibitor S3I-201 and the TGFβ1 160 specific inhibitor SB431542 were purchased from Merck Life Science 161 (Shanghai, China).

    Article Title: Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
    Article Snippet: Peptide, recombinant protein , Recombinant human IL-6 , R and D systems , Cat# 206-IL-010 , .

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes.
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100μg/ml and stored at −20C.

    Generated:

    Article Title: GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells
    Article Snippet: Transfected cells were cultured with recombinant human IL-6 (#206-IL-050, R&D) (10 ng/mL) for 12 h or 48 h.

    Article Title: IL-27 Is Elevated in Patients With COPD and Patients With Pulmonary TB and Induces Human Bronchial Epithelial Cells to Produce CXCL10
    Article Snippet: Recombinant human IL-1β, IL-4, IL-6, IL-12, IL-13, IL-17F, IL-23, IL-27, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were purchased from R&D Systems.

    Article Title: Antioxidant inflammation modulators: oleanolic acid derivatives with amino and other modifications at C-17
    Article Snippet: Lysates were immunoblotted with antibodies against phosphorylated or total STAT3 (Cell Signaling). iNOS Induction qPCR.

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100 μg/ml and stored at −20C.

    Article Title: Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.
    Article Snippet: From the University of California San Diego human research protection program) according to the protocol by Kirshenbaum and Metcalfe.59 Briefly, CD34+ cells were cultured in serum-free culture media (Stemline II, Sigma) containing antibiotic-antimycotic (Thermo Fisher), recombinant human stem cell factor (100 ng/ml, R&D Systems), recombinant human IL-6 (100 ng/ml, R&D Systems), and recombinant human IL-3 (20 ng/ml, R&D Systems, first week only).

    Article Title: IL-6 trans- signaling drives a STAT3-dependent pathway that leads to structural alterations of the peritoneal membrane.
    Article Snippet: Recombinant human IL-6, soluble IL-6 receptor (sIL-6R) and 158 soluble gp130 (sgp130) were purchased from R&D Systems 159 (Minneapolis, MN).The STAT3 specific inhibitor S3I-201 and the TGFβ1 160 specific inhibitor SB431542 were purchased from Merck Life Science 161 (Shanghai, China).

    Article Title: Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
    Article Snippet: Peptide, recombinant protein , Recombinant human IL-6 , R and D systems , Cat# 206-IL-010 , .

    Article Title: TLR3 activation mediates partial epithelial-to-mesenchymal transition in human keratinocytes.
    Article Snippet: Recombinant human IL-6 (R&D) was made in sterile PBS at 100μg/ml and stored at −20C.



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    Network pharmacology and molecular docking analysis of loganin-associated targets in UC. ( A ) Venn diagram showing the overlap between loganin-related targets and UC-associated genes. ( B ) Protein-protein interaction (PPI) network of the overlapping targets, highlighting key hub genes involved in loganin’s potential mechanisms, including TNF, EGFR, IL6, STAT3, and BCL2. ( C ) Molecular docking of loganin with IL-6. ( D ) Molecular docking of loganin with STAT3.

    Journal: Scientific Reports

    Article Title: Loganin alleviates oxidative stress-induced apoptosis by modulating mitochondrial function and STAT3 signaling in DSS-induced colitis and H 2 O 2 -injured Caco-2 cells

    doi: 10.1038/s41598-026-55447-3

    Figure Lengend Snippet: Network pharmacology and molecular docking analysis of loganin-associated targets in UC. ( A ) Venn diagram showing the overlap between loganin-related targets and UC-associated genes. ( B ) Protein-protein interaction (PPI) network of the overlapping targets, highlighting key hub genes involved in loganin’s potential mechanisms, including TNF, EGFR, IL6, STAT3, and BCL2. ( C ) Molecular docking of loganin with IL-6. ( D ) Molecular docking of loganin with STAT3.

    Article Snippet: To establish the oxidative stress model, Caco-2 cells were exposed to H 2 O 2 (100 μM) for 12 h. For interleukin-6 (IL-6) supplementation experiments, recombinant human IL-6 (MedChemExpress, HY-P7044) was added at 10 ng/mL for 24 h in the H 2 O 2 + loganin group before subsequent analyses, with reference to previous studies using IL-6 stimulation in Caco-2 cells .

    Techniques:

    Loganin inhibits apoptosis through the IL-6/STAT3/BCL2 signaling pathway in H 2 O 2 -induced epithelial cell injury. ( A ) Western blot analysis of apoptosis-related proteins Bcl-2, BAX, pro-caspase-3, and cleaved caspase-3. ( B – D ) Quantification of BAX, Bcl-2, and cleaved caspase-3 protein levels. ( E ) Western blot analysis of STAT3 and p-STAT3 protein levels. ( F ) Quantification of p-STAT3 protein levels. ( G ) Flow cytometry analysis of apoptosis in Ctrl, H 2 O 2 , H 2 O 2 + loganin, and H 2 O 2 + loganin + IL-6 groups. ( H ) Quantification of apoptosis rate. ( I ) Western blot analysis of apoptosis-related proteins Bcl-2, BAX, pro-caspase-3, and cleaved caspase-3 in Ctrl, H 2 O 2 , H 2 O 2 + loganin, and H 2 O 2 + loganin + IL-6 groups. ( J – L ) Quantification of BAX, Bcl-2, and cleaved caspase-3 protein levels, respectively. ( M ) Western blot analysis of STAT3 and p-STAT3 protein levels. ( N ) Quantification of p-STAT3 protein levels. Data are presented as mean ± SD ( n = 4 per group). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; ns, not significant. For panels A–F, H 2 O 2 was compared with Ctrl, H 2 O 2 + loganin was compared with H 2 O 2 , and the loganin group was compared with Ctrl. For panels G–N, H 2 O 2 was compared with Ctrl, H 2 O 2 + loganin was compared with H 2 O 2 , and H 2 O 2 + loganin + IL-6 was compared with H 2 O 2 + loganin.

    Journal: Scientific Reports

    Article Title: Loganin alleviates oxidative stress-induced apoptosis by modulating mitochondrial function and STAT3 signaling in DSS-induced colitis and H 2 O 2 -injured Caco-2 cells

    doi: 10.1038/s41598-026-55447-3

    Figure Lengend Snippet: Loganin inhibits apoptosis through the IL-6/STAT3/BCL2 signaling pathway in H 2 O 2 -induced epithelial cell injury. ( A ) Western blot analysis of apoptosis-related proteins Bcl-2, BAX, pro-caspase-3, and cleaved caspase-3. ( B – D ) Quantification of BAX, Bcl-2, and cleaved caspase-3 protein levels. ( E ) Western blot analysis of STAT3 and p-STAT3 protein levels. ( F ) Quantification of p-STAT3 protein levels. ( G ) Flow cytometry analysis of apoptosis in Ctrl, H 2 O 2 , H 2 O 2 + loganin, and H 2 O 2 + loganin + IL-6 groups. ( H ) Quantification of apoptosis rate. ( I ) Western blot analysis of apoptosis-related proteins Bcl-2, BAX, pro-caspase-3, and cleaved caspase-3 in Ctrl, H 2 O 2 , H 2 O 2 + loganin, and H 2 O 2 + loganin + IL-6 groups. ( J – L ) Quantification of BAX, Bcl-2, and cleaved caspase-3 protein levels, respectively. ( M ) Western blot analysis of STAT3 and p-STAT3 protein levels. ( N ) Quantification of p-STAT3 protein levels. Data are presented as mean ± SD ( n = 4 per group). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; ns, not significant. For panels A–F, H 2 O 2 was compared with Ctrl, H 2 O 2 + loganin was compared with H 2 O 2 , and the loganin group was compared with Ctrl. For panels G–N, H 2 O 2 was compared with Ctrl, H 2 O 2 + loganin was compared with H 2 O 2 , and H 2 O 2 + loganin + IL-6 was compared with H 2 O 2 + loganin.

    Article Snippet: To establish the oxidative stress model, Caco-2 cells were exposed to H 2 O 2 (100 μM) for 12 h. For interleukin-6 (IL-6) supplementation experiments, recombinant human IL-6 (MedChemExpress, HY-P7044) was added at 10 ng/mL for 24 h in the H 2 O 2 + loganin group before subsequent analyses, with reference to previous studies using IL-6 stimulation in Caco-2 cells .

    Techniques: Western Blot, Flow Cytometry